Analysis of honey using capillary electrophoresis with electrokinetic injection - Direct analysis of viscous samples without sample pretreatment.

ABSTRACT

Although the electrokinetic injection mode is applicable for direct introduction of ionic components from highly viscous samples, this is the first work which studies this special feature of capillary electrophoresis for this purpose. 9 metal cations were determined in honey utilizing direct injection, zone electrophoretic separation and indirect UV detection of the sample components. Reproducibility better than 4 RSD% was found for peak areas and the LOD values ranged between 0.045 and 0.44 mg/kg when injection parameters were set to 7.5 kV × 5 s. In order to remedy the quantitation problems arising from mobility and matrix bias, the applicability of the internal universal calibration was examined. It was found that in cases where the mobilities of cations do not differ from the mobilities of the monitoring ion and the internal universal standard (IUS) by more than 10%, the obtained results agreed well with the data measured with ICP-OES.