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Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals.
Liu, Jia; Kim, Yoon Seok; Richardson, Claire E; Tom, Ariane; Ramakrishnan, Charu; Birey, Fikri; Katsumata, Toru; Chen, Shucheng; Wang, Cheng; Wang, Xiao; Joubert, Lydia-Marie; Jiang, Yuanwen; Wang, Huiliang; Fenno, Lief E; Tok, Jeffrey B-H; Pașca, Sergiu P; Shen, Kang; Bao, Zhenan; Deisseroth, Karl.
Afiliação
  • Liu J; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.
  • Kim YS; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
  • Richardson CE; Department of Biology, Stanford University, Stanford, CA 94305, USA.
  • Tom A; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
  • Ramakrishnan C; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
  • Birey F; Department of Psychiatry, Stanford University, Stanford, CA 94305, USA.
  • Katsumata T; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.
  • Chen S; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.
  • Wang C; Advanced Light Source, Lawrence Berkeley National Laboratory, Berkeley CA 94720, USA.
  • Wang X; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
  • Joubert LM; Cell Sciences Imaging Facility, Stanford University, Stanford, CA 94305, USA.
  • Jiang Y; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.
  • Wang H; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
  • Fenno LE; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
  • Tok JB; Department of Psychiatry, Stanford University, Stanford, CA 94305, USA.
  • Pașca SP; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.
  • Shen K; Department of Psychiatry, Stanford University, Stanford, CA 94305, USA.
  • Bao Z; Department of Biology, Stanford University, Stanford, CA 94305, USA.
  • Deisseroth K; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Science ; 367(6484): 1372-1376, 2020 03 20.
Article em En | MEDLINE | ID: mdl-32193327
ABSTRACT
The structural and functional complexity of multicellular biological systems, such as the brain, are beyond the reach of human design or assembly capabilities. Cells in living organisms may be recruited to construct synthetic materials or structures if treated as anatomically defined compartments for specific chemistry, harnessing biology for the assembly of complex functional structures. By integrating engineered-enzyme targeting and polymer chemistry, we genetically instructed specific living neurons to guide chemical synthesis of electrically functional (conductive or insulating) polymers at the plasma membrane. Electrophysiological and behavioral analyses confirmed that rationally designed, genetically targeted assembly of functional polymers not only preserved neuronal viability but also achieved remodeling of membrane properties and modulated cell type-specific behaviors in freely moving animals. This approach may enable the creation of diverse, complex, and functional structures and materials within living systems.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenilenodiaminas / Polímeros / Engenharia Genética / Ascorbato Peroxidases / Compostos de Anilina / Neurônios / Nitrocompostos Limite: Animals / Humans Idioma: En Revista: Science Ano de publicação: 2020 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenilenodiaminas / Polímeros / Engenharia Genética / Ascorbato Peroxidases / Compostos de Anilina / Neurônios / Nitrocompostos Limite: Animals / Humans Idioma: En Revista: Science Ano de publicação: 2020 Tipo de documento: Article