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A RuO2 Nanosheet as a Novel Quencher-free Platform for the Detection of Nucleic Acids in a Homogeneous Solution.
Kitamura, Yusuke; Taniguchi, Takaaki; Tsutsumi, Miwako; Nurdiwijayanto, Leanddas; Matsuo, Tomoya; Katsuda, Yousuke; Ihara, Toshihiro.
Afiliação
  • Kitamura Y; Division of Materials Science, Faculty of Advanced Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo, Kumamoto, 860-8555, Japan. ykita@kumamoto-u.ac.jp.
  • Taniguchi T; World Premier International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
  • Tsutsumi M; Division of Materials Science, Faculty of Advanced Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo, Kumamoto, 860-8555, Japan.
  • Nurdiwijayanto L; World Premier International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
  • Matsuo T; Division of Materials Science, Faculty of Advanced Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo, Kumamoto, 860-8555, Japan.
  • Katsuda Y; Division of Materials Science, Faculty of Advanced Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo, Kumamoto, 860-8555, Japan.
  • Ihara T; Division of Materials Science, Faculty of Advanced Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo, Kumamoto, 860-8555, Japan. toshi@chem.kumamoto-u.ac.jp.
Anal Sci ; 36(4): 397-400, 2020 Apr 10.
Article em En | MEDLINE | ID: mdl-32201407
ABSTRACT
A fluorescent dye-labeled DNA probe was adsorbed and quenched on the monolayer of RuO2 nanosheets. Significant fluorescent recovery was observed upon the addition of complementary DNA due to desorption of the probe from the surface of the RuO2 nanosheet through duplex formation. The efficiency of fluorescence recovery was higher than that for graphene oxide, which was known as a quencher-free platform for the detection of nucleic acids in a homogeneous solution.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Sondas de DNA / Compostos de Rutênio / Nanoestruturas / Corantes Fluorescentes Tipo de estudo: Diagnostic_studies Idioma: En Revista: Anal Sci Ano de publicação: 2020 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Sondas de DNA / Compostos de Rutênio / Nanoestruturas / Corantes Fluorescentes Tipo de estudo: Diagnostic_studies Idioma: En Revista: Anal Sci Ano de publicação: 2020 Tipo de documento: Article