Dimensionality reduction by UMAP to visualize physical and genetic interactions.
Nat Commun
; 11(1): 1537, 2020 03 24.
Article
em En
| MEDLINE
| ID: mdl-32210240
ABSTRACT
Dimensionality reduction is often used to visualize complex expression profiling data. Here, we use the Uniform Manifold Approximation and Projection (UMAP) method on published transcript profiles of 1484 single gene deletions of Saccharomyces cerevisiae. Proximity in low-dimensional UMAP space identifies groups of genes that correspond to protein complexes and pathways, and finds novel protein interactions, even within well-characterized complexes. This approach is more sensitive than previous methods and should be broadly useful as additional transcriptome datasets become available for other organisms.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Algoritmos
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Perfilação da Expressão Gênica
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Proteínas de Saccharomyces cerevisiae
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Mapeamento de Interação de Proteínas
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Nat Commun
Ano de publicação:
2020
Tipo de documento:
Article