Investigating the synergic effects of valproic acid and crocin on BDNF and GDNF expression in epidermal neural crest stem cells.
Acta Neurobiol Exp (Wars)
; 80(1): 38-46, 2020.
Article
em En
| MEDLINE
| ID: mdl-32214273
ABSTRACT
Following nerve tissue damage, various events, such as inflammatory responses, microglial activation, endoplasmic reticulum stress, and apoptosis, can occur, which all lead to cell death, prevent axonal growth, and cause axonal circumvolution. So far, several researchers have tended to adopt strategies to reduce the harmful conditions associated with neurological disorders, and stemcellbased therapy is one of those promising strategies. Epidermal neural crest stem cells (EPINCSCs) are a type of stem cell that has widely been employed for the treatment of various neurological disorders. It has been suggested that these stem cells perform their supportive actions primarily through the release of different neurotrophic factors. Hence, in this study, the neuroprotective impacts of valproic acid (VPA) and crocin were evaluated on the mRNA expression levels of brainderived neurotrophic factor (BDNF) and glialcellderived neurotrophic factor (GDNF) in EPINCSCs. In this research, we isolated EPINCSCs from the hair follicles of the rat whisker pad. Then, the cells were treated with different concentrations of VPA and crocin for 72 h. Subsequently, an MTT assay was performed to define the suitable concentrations of drugs. Finally, realtime PCR was performed to evaluate the mRNA expression levels of BDNF and GDNF in these stem cells. The results of the MTT assay showed that the treatment of EPINCSCs with 1 mM VPA and 1.5 mM crocin, and the cotreatment with 1 mM VPA and 500 µM crocin, led to the survival and proliferation of these stem cells. Moreover, the realtime PCR results revealed that both VPA and crocin, both individually and in combination, can significantly increase the expression levels of BDNF and GDNF in EPINCSCs. According to the findings of this study, both VPA and crocin, alone and in combination, are potential candidates for enhancing the capacity of EPINCSCs to differentiate into neural lineages. Therefore, the cotreatment of EPINCSCs with these drugs can be employed for the treatment of various neurological disorders, such as spinal cord injury.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carotenoides
/
Regulação da Expressão Gênica
/
Ácido Valproico
/
Fator Neurotrófico Derivado do Encéfalo
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Fator Neurotrófico Derivado de Linhagem de Célula Glial
/
Crista Neural
Limite:
Animals
Idioma:
En
Revista:
Acta Neurobiol Exp (Wars)
Ano de publicação:
2020
Tipo de documento:
Article