Tetrazolo[1,5-a]quinoline moiety-based Os(IV) complexes: DNA binding/cleavage, bacteriostatic and photocytotoxicity assay.

ABSTRACT

Biological applications of platinum group metal-based complexes have been widely explored in synthetic and inorganic chemistry. The compounds have been subjected to DNA binding, DNA cleavage, In-vivo and In-vitro photocytotoxicity (HCT-116 cell line) and bacteriostatic activities. Binding constant of complexes are 1.42-5.62 × 104 M-1, whereas that of ligands are 1.12-4.72 × 104 M-1. Ksv of complexes are about 1.32-5.21 × 103 M-1, whereas Kf is about 1.24-6.83 × 103 M-1. IC50 of compounds screened using HCT-116 cell line in dark are found to be 121-342 µg/mL. Whereas photocytotoxicity is found in the range of 48-316 µg/mL. Docking energy of molecules have been evaluated to evaluate efficacy of binding. Molecular docking energy of complexes are in the range of -286.00 to -303.11 kJ/mol. Whereas that of ligands are -254.03 to -282.96 kJ/mol. MIC of complexes are 47 ± 2.5 to 77.50 ± 7.5 µM. LC50 values of ligands fall in the range of 4.05-19.72 µg/mL and that of Os(IV) complexes fall in the range of 3.99-15.99 µg/mL. The Os(IV) complexes dominate in proving its potentiality compared to N, N-donor ligands in biological activities. [Formula see text]Communicated by Ramaswamy H. Sarma.