Treadmill exercise promotes neurogenesis and myelin repair via upregulating Wnt/ßcatenin signaling pathways in the juvenile brain following focal cerebral ischemia/reperfusion.
Int J Mol Med
; 45(5): 1447-1463, 2020 May.
Article
em En
| MEDLINE
| ID: mdl-32323740
ABSTRACT
Physical exercise has a neuroprotective effect and is an important treatment after ischemic stroke. Promoting neurogenesis and myelin repair in the penumbra is an important method for the treatment of ischemic stroke. However, the role and potential mechanism of exercise in neurogenesis and myelin repair still needs to be clarified. The goal of the present study was to ascertain the possible effect of treadmill training on the neuroprotective signaling pathway in juvenile rats after ischemic stroke. The model of middle cerebral artery occlusion (MCAO) in juvenile rats was established and then the rats were randomly divided into 9 groups. XAV939 (an inhibitor of the Wnt/ßcatenin pathway) was used to confirm the effects of the Wnt/ßcatenin signaling pathway on exercisemediated neurogenesis and myelin repair. Neurological deficits were detected by modified neurological severity score, the injury of brain tissue and the morphology of neurons was detected by hematoxylineosin staining and Nissl staining, and the infarct volume was detected by 2,3,5triphenyl tetrazolium chloride staining. The changes in myelin were observed by Luxol fast blue staining. The neuron ultrastructure was observed by transmission electron microscopy. Immunofluorescence and western blots analyzed the molecular mechanisms. The results showed that treadmill exercise improved neurogenesis, enhanced myelin repair, promoted neurological function recovery and reduced infarct volume. These were the results of the upregulation of Wnt3a and nucleus ßcatenin, brainderived neurotrophic factor (BDNF) and myelin basic protein (MBP). In addition, XAV939 inhibited treadmill exerciseinduced neurogenesis and myelin repair, which was consistent with the downregulation of Wnt3a, nucleus ßcatenin, BDNF and MBP expression, and the deterioration of neurological function. In summary, treadmill exercise promotes neurogenesis and myelin repair by upregulating the Wnt/ßcatenin signaling pathway, to improve the neurological deficit caused by focal cerebral ischemia/reperfusion.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Condicionamento Físico Animal
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Encéfalo
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Regulação para Cima
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Isquemia Encefálica
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Beta Catenina
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Neurogênese
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Via de Sinalização Wnt
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Bainha de Mielina
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Int J Mol Med
Ano de publicação:
2020
Tipo de documento:
Article