Enzymatic epoxidation of cyclohexene by peroxidase immobilization on a textile and an adapted reactor design.
Enzyme Microb Technol
; 136: 109512, 2020 May.
Article
em En
| MEDLINE
| ID: mdl-32331717
ABSTRACT
A textile-based reaction system for new peroxidase reactions in non-native media was implemented. The epoxidation of cyclohexene by the commercial peroxidase MaxiBright® was realized with the textile-immobilized enzyme in an adapted liquid-liquid two-phase reactor. A commercially available polyester felt was used as low-price carrier and functionalized with polyvinyl amine. The covalent immobilization with glutardialdehyde lead to an enzyme loading of 0.10 genzyme/gtextile. The textile-based peroxidase shows a high activity retention in the presence of organic media. This catalyst is shown to enable the epoxidation of cyclohexene in various solvents as well as under neat conditions. A model reactor was produced by 3D printing which places the textile catalyst at the interphase between the liquid reaction phase and the product extracting solvent.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peroxidases
/
Têxteis
/
Enzimas Imobilizadas
/
Cicloexenos
Idioma:
En
Revista:
Enzyme Microb Technol
Ano de publicação:
2020
Tipo de documento:
Article