Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform.
Commun Biol
; 3(1): 236, 2020 05 14.
Article
em En
| MEDLINE
| ID: mdl-32409770
ABSTRACT
Tuberculosis (TB) remains a leading cause of death worldwide. Lipid rich, phenotypically antibiotic tolerant, bacteria are more resistant to antibiotics and may be responsible for relapse and the need for long-term TB treatment. We present a microfluidic system that acoustically traps live mycobacteria, M. smegmatis, a model organism for M. tuberculosis. We then perform optical analysis in the form of wavelength modulated Raman spectroscopy (WMRS) on the trapped M. smegmatis for up to eight hours, and also in the presence of isoniazid (INH). The Raman fingerprints of M. smegmatis exposed to INH change substantially in comparison to the unstressed condition. Our work provides a real-time assessment of the impact of INH on the increase of lipids in these mycobacteria, which could render the cells more tolerant to antibiotics. This microfluidic platform may be used to study any microorganism and to dynamically monitor its response to different conditions and stimuli.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Análise Espectral Raman
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Mycobacterium smegmatis
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Microfluídica
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Isoniazida
Idioma:
En
Revista:
Commun Biol
Ano de publicação:
2020
Tipo de documento:
Article