DNA Capture by Nanopore Sensors under Flow.
Anal Chem
; 92(12): 8108-8116, 2020 06 16.
Article
em En
| MEDLINE
| ID: mdl-32412730
ABSTRACT
Integrating nanopore sensors within microfluidic architectures is key to providing advanced sample processing capabilities upstream of the biosensor. When confined in a microchannel, the nanopore capture and translocation characteristics are altered when subjected to cross-flow, affecting sensor performance. Here, we study the capture rate and translocation of 1-5 kbp double-stranded DNA molecules through solid-state nanopores in the presence of tangential fluid flow over the nanopore aperture. Experiments reveal a trend of increased capture rate with cross-flow, reaching a 5-fold enhancement (dependent on DNA length) at moderate flow rates, before decreasing at higher flow rates. By modeling DNA dynamics in microchannels under the combined effect of laminar flow, Brownian motion and electrophoretic drift, it is shown that the observed trend is the result of two competing mechanisms enhanced DNA transport by convection and reduction in the nanopore's capture volume with increased flow velocity. Moreover, it is shown that the viscous drag force exerted by flow on a translocating DNA can be exploited to tune the kinetics of DNA translocation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Dispositivos Lab-On-A-Chip
/
Nanoporos
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2020
Tipo de documento:
Article