Automation of RNA-based biomarker extraction from dried blood spots for the detection of blood doping.
Bioanalysis
; 12(11): 729-736, 2020 Jun.
Article
em En
| MEDLINE
| ID: mdl-32412781
ABSTRACT
Aim:
Transcriptomic biomarkers originating from reticulocytes measured in dried blood spots (DBSs) may be reliable indicators of blood doping. Methods/results:
Here, we examined changes in the expression levels of the erythropoiesis-related ALAS2, CA1 and SLC4A1 genes in DBS samples from elite athletes and volunteers of clinical study with recombinant erythropoietin dose.Conclusion:
By comparing the mean intraday coefficients of variation for ALAS2L, ALASLC, CA1 and SLC4A1 between manual and automated RNA extractions, an average improvement was observed, whereas the assessment of interday variability provided comparable results for both manual and automated approaches. Our results confirmed that RNA biomarkers on DBS support are efficient to detect blood doping.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Automação
/
RNA
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Detecção do Abuso de Substâncias
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Dopagem Esportivo
/
Teste em Amostras de Sangue Seco
Limite:
Humans
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2020
Tipo de documento:
Article