SRT1720-induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA-dependent phosphorylation of AMPKα at Ser485.
FEBS Open Bio
; 10(7): 1316-1325, 2020 07.
Article
em En
| MEDLINE
| ID: mdl-32421926
ABSTRACT
Aging is a major risk factor for hypertension and atherosclerosis, and vascular smooth muscle cell (VSMC) senescence can promote aging-related vascular diseases. Sirtuin-1 (SIRT1) and AMP-activated protein kinase (AMPK) were previously reported to modulate vascular senescence; however, its effects have not been well characterized. To determine the nature of the interaction between SIRT1 and AMPK in VSMC senescence, we investigated the effects of SRT1720 on its downstream targets of SIRT1 and the phosphorylation of AMPKα at Ser485. During Adriamycin-induced VSMC senescence, SRT1720 increased the activity of SIRT1 and AMPKα phosphorylation at Ser485 via the cAMP-protein kinase A (PKA) pathway. Telomere length and telomerase reverse transcriptase expression were increased by SIRT1 activation with SRT1720. Taken together, these data show that activation of the SIRT1/cAMP-PKA/p-AMPKα (Ser485) pathway may be an effective antisenescence mechanism for VSMCs.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Serina
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Proteínas Quinases Dependentes de AMP Cíclico
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Proteínas Quinases Ativadas por AMP
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Sirtuína 1
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Compostos Heterocíclicos de 4 ou mais Anéis
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Músculo Liso Vascular
Limite:
Animals
Idioma:
En
Revista:
FEBS Open Bio
Ano de publicação:
2020
Tipo de documento:
Article