Generation of Footprint-Free Canine Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus Vector.

Tsukamoto, Masaya; Kimura, Kazuto; Tanaka, Miyuu; Kuwamura, Mitsuru; Ohtaka, Manami; Nakanishi, Mahito; Sugiura, Kikuya; Hatoya, Shingo

Tsukamoto, Masaya; Kimura, Kazuto; Tanaka, Miyuu; Kuwamura, Mitsuru; Ohtaka, Manami; Nakanishi, Mahito; Sugiura, Kikuya; Hatoya, Shingo.

Afiliação

Tsukamoto M; Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
Kimura K; Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
Tanaka M; Department of Integrated Structural Biosciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
Kuwamura M; Department of Integrated Structural Biosciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
Ohtaka M; TOKIWA-Bio, Inc., Tsukuba, Japan.
Nakanishi M; TOKIWA-Bio, Inc., Tsukuba, Japan.
Sugiura K; Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Hatoya S; Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.

Mol Reprod Dev ; 87(6): 663-665, 2020 06.

Article em En | MEDLINE | ID: mdl-32424848

ABSTRACT

ABSTRACT

Using auto-erasable Sendai virus vector, we generated ciPSC line. After several passages, virus was not present in ciPSCs by RT-PCR. ciPSCs from canine PBMCs had pluripotent state, differentiated all three germ layers in vitro, and had normal 78 XX karyotype. These results proved that PBMCs were one of the good cell sources to generate ciPSC lines from companion and patient dogs.

Assuntos

Cães; Células-Tronco Pluripotentes Induzidas/fisiologia; Leucócitos Mononucleares/fisiologia; Cultura Primária de Células; Vírus Sendai/fisiologia; Animais; Diferenciação Celular/genética; Linhagem Celular Transformada; Transformação Celular Viral/genética; Reprogramação Celular/genética; Feminino; Vetores Genéticos/genética; Células-Tronco Pluripotentes Induzidas/citologia; Cariótipo; Leucócitos Mononucleares/citologia; Cultura Primária de Células/métodos; Cultura Primária de Células/veterinária; Vírus Sendai/genética

Palavras-chave

SeVdp(KOSM)302L; Sendai virus vector; canine induced pluripotent stem cells; peripheral blood mononuclear cells

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucócitos Mononucleares / Vírus Sendai / Cães / Células-Tronco Pluripotentes Induzidas / Cultura Primária de Células Limite: Animals Idioma: En Revista: Mol Reprod Dev Ano de publicação: 2020 Tipo de documento: Article

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