Ecofriendly chromatographic methods for determination of co-prescribed drugs, olanzapine and metformin, in rat plasma.
Bioanalysis
; 12(9): 597-613, 2020 May.
Article
em En
| MEDLINE
| ID: mdl-32441535
ABSTRACT
Background:
Olanzapine (OLZ) is one of most recommended drugs for the treatment of schizophrenia while metformin (MET) is the most commonly used hypoglycemic agent.Aim:
Development and validation of two green, sensitive and accurate chromatographic methods for the simultaneous determination of OLZ along with the co-prescribed, MET. Materials &methods:
TLC-densitometric method with a developing system consisting of methylene chloridemethanolethyl acetatetriethylamine (4450.1, by volume) and a reversed-phase (RP)-HPLC method where the chromatographic separation was performed using ethanolwater mixture (50 50, v/v) as a mobile phase.Results:
TLC-densitometric method had linearity over concentration ranges of 160-4000 ng/band for OLZ and 150-4500 ng/band for MET, while RP-HPLC method was linear and validated over concentration range of 300-20000 ng/ml for OLZ and MET.Conclusion:
Pharmacokinetic study was successfully performed and suggested the possibility of co-administration of MET with OLZ and their further formulation in one pharmaceutical preparation to enhance patient's compliance.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Olanzapina
/
Metformina
Limite:
Animals
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2020
Tipo de documento:
Article