Purification of Fusion Proteins by Affinity Chromatography on Glutathione Resin.
Cold Spring Harb Protoc
; 2020(6): 102202, 2020 06 01.
Article
em En
| MEDLINE
| ID: mdl-32482903
ABSTRACT
Fusion proteins that contain a glutathione S-transferase (GST) moiety can be purified to near homogeneity by affinity chromatography on glutathione-linked resins. Glutathione immobilized on a chromatography matrix, such as agarose or Sepharose, acts as a substrate for the GST moiety of fusion proteins. Contaminating proteins are washed away, and the bound GST fusion proteins are then readily displaced from the resin by elution with buffers containing free glutathione.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Resinas Sintéticas
/
Proteínas Recombinantes de Fusão
/
Cromatografia de Afinidade
/
Glutationa
/
Glutationa Transferase
Idioma:
En
Revista:
Cold Spring Harb Protoc
Ano de publicação:
2020
Tipo de documento:
Article