CD31 (PECAM-1) Serves as the Endothelial Cell-Specific Receptor of Clostridium perfringens ß-Toxin.
Cell Host Microbe
; 28(1): 69-78.e6, 2020 07 08.
Article
em En
| MEDLINE
| ID: mdl-32497498
ABSTRACT
Clostridium perfringens ß-toxin (CPB) is a highly active ß-pore-forming toxin (ß-PFT) and the essential virulence factor for fatal, necro-hemorrhagic enteritis in animals and humans. The molecular mechanisms involved in CPB's action on its target, the endothelium of small intestinal vessels, are poorly understood. Here, we identify platelet endothelial cell adhesion molecule-1 (CD31 or PECAM-1) as the specific membrane receptor for CPB on endothelial cells. CD31 expression corresponds with the cell-type specificity of CPB, and it is essential for toxicity in cultured cells and mice. Ectopic CD31 expression renders resistant cells and liposomes susceptible to CPB-induced membrane damage. Moreover, the extracellular Ig6 domain of mouse, human, and porcine CD31 is essential for the interaction with CPB. Hence, our results explain the cell-type specificity of CPB in vitro and in the natural disease caused by C. perfringens type C.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Toxinas Bacterianas
/
Clostridium perfringens
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Molécula-1 de Adesão Celular Endotelial a Plaquetas
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Células Endoteliais
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
Cell Host Microbe
Ano de publicação:
2020
Tipo de documento:
Article