A New Protocol for the Detection of Sterigmatocystin-producing Aspergillus Section Versicolores Using a High Discrimination Polymerase.
Biocontrol Sci
; 25(2): 113-118, 2020.
Article
em En
| MEDLINE
| ID: mdl-32507789
ABSTRACT
Aspergillus section Versicolores species, except Aspergillus sydowii, produce a carcinogenic mycotoxin sterigmatocystin (STC). Since these fungi are found in varied environmental milieu including indoor dust and food products, our aim was to develop a sensitive and convenient assay to detect STC producing fungal strains. We made use of a high discrimination DNA polymerase (HiDi DNA polymerase), for single nucleotide polymorphism (SNP)-based PCR amplification. Using specific primer pairs based on the SNPs between A. sydowii and other strains of Aspergillus section Versicolores, we succeeded in amplifying the genomic DNA all target strains except A. sydowii. These results confirm that the SNP-based PCR amplification technique, using a high discrimination DNA polymerase, was a reliable and robust screening method for target fungal strains.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aspergillus
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Proteínas Fúngicas
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Regulação Fúngica da Expressão Gênica
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Reação em Cadeia da Polimerase
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Polimorfismo de Nucleotídeo Único
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DNA Polimerase Dirigida por DNA
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Idioma:
En
Revista:
Biocontrol Sci
Ano de publicação:
2020
Tipo de documento:
Article