Characterization of SepE and SepF for the N6-Glycosylated Adenine Structure Formation in Septacidin Biosynthesis.
Org Lett
; 22(13): 5251-5254, 2020 07 02.
Article
em En
| MEDLINE
| ID: mdl-32567317
ABSTRACT
Septacidin (1) represents a group of nucleoside antibiotics possessing a unique N6-glycosylated adenine core. They exhibit some fascinating bioactivities that are rare for other nucleoside antibiotics. Here we demonstrate that this unique structure in septacidin is formed by SepE and SepF. SepE is an unprecedented Fe(II)-dependent glycosyltransferase decorating the N6-position of AMP using ADP-l-glycero-ß-d-manno-heptose (6) as a sugar donor. The Fe(II) may help SepE to bind AMP. SepF is an unusual glycosidase that detaches the N9-ribosyl-5-phosphate.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Adenina
/
Glicosídeo Hidrolases
Idioma:
En
Revista:
Org Lett
Ano de publicação:
2020
Tipo de documento:
Article