The Epstein-Barr virus-encoded G protein-coupled receptor BILF1 upregulates ICAM-1 through a mechanism involving the NF-ÒB pathway.
Biosci Biotechnol Biochem
; 84(9): 1810-1819, 2020 Sep.
Article
em En
| MEDLINE
| ID: mdl-32567483
ABSTRACT
Although the Epstein-Barr virus (EBV) infection is usually asymptomatic, a primary encounter with the virus can cause mononucleosis. EBV infection is also strongly associated with lymphoma and epithelial cancers. The structure and infection mechanism of EBV have been well studied, but the EBV-encoded G protein-coupled receptor, BILF1, is not fully understood. Here, it was found that the EBV BILF1 was expressed early in the viral lytic cycle and its ectopic expression strikingly upregulated the ICAM-1 expression in Raji cells. The positive effect of BILF1 on the ICAM-1 promoter was observed and the BILF1 deficiency attenuated ICAM-1 promoter activity. Moreover, NF-κB binding sites were important for the regulation of ICAM-1 promoter by BILF1. Furthermore, BILF1 reduced the constitutive level of the IÒB-a protein and increased the amount of nuclear NF-ÒB in Raji cells. In conclusion, this study determined that BILF1 upregulated ICAM-1 in a mechanism involving NF-ÒB.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
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Transdução de Sinais
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Regulação para Cima
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NF-kappa B
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Herpesvirus Humano 4
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Molécula 1 de Adesão Intercelular
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Receptores Acoplados a Proteínas G
Limite:
Humans
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Ano de publicação:
2020
Tipo de documento:
Article