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Analysis of Inflammatory and Homeostatic Roles of Tissue-resident Macrophages in the Progression of Cholesteatoma by RNA-Seq.
Fang, Lian; Chen, Lin; Lin, Bi; Han, Liang; Zhu, Kaiquan; Song, Qifa.
Afiliação
  • Fang L; First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
  • Chen L; Department of Pathology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
  • Lin B; First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
  • Han L; First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
  • Zhu K; First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
  • Song Q; Central Laboratory, Ningbo First Hospital, Ningbo City, Zhejiang Province, China.
Immunol Invest ; 50(6): 609-621, 2021 Aug.
Article em En | MEDLINE | ID: mdl-32573304
ABSTRACT

BACKGROUND:

Tissue-resident macrophages (TRMØs) can act as innate-immune sentinels to protect body against microbe invaders and stimulating materials such as cholesterol crystals in cholesteatoma, as well as to preserve tissue integrity by cleaning unwanted cellular debris.

METHODS:

TRMØs in the incised middle ear tissues were obtained from the patients with cholesteatoma as an experimental group and the patients without cholesteatoma as a control group. Differential gene expression profiling of TRMØs was conducted between two groups by analyzing GO processes, KEGG and GSEA pathways of inflammation, tissue repair and homeostasis.

RESULTS:

The current study showed that 145 of 7060 genes were significantly up-regulated (logFC>2 and FDR <0.05) when compared with the patients without cholesteatoma. GO process, GSEA and Cytoscape analysis of the over-expressed genes illustrated the boosted inflammatory and anti-infection functions of TRMØs existed neutrophil function, leukocyte migration, and adaptive immune response involved receptors and signaling pathways. Whereas the homeostasis and repair functions of TRMØs were affected from up-regulated genes, such as over-expressed keratin-13 that helped form the outer keratinising squamous epithelial layer, and over-expressed MMPs that activated the extracellular matrix molecules to promote inflammation and disturb tissue remodeling. Additionally, 74 down-regulated genes (logFC<-2 and FDR <0.05) also affected the homeostasis and repair functions by affecting extracelluar matrix structure and contractile fibres in TRMØs.

CONCLUSIONS:

The cellular and molecular levels in cholesteatoma is attributable to chronic infection and several disturbed cellular biological processes involving cell integrity and tissue remodeling.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regulação da Expressão Gênica / Colesteatoma da Orelha Média / Infecção Persistente / Macrófagos Tipo de estudo: Observational_studies Limite: Adult / Aged / Humans / Male / Middle aged Idioma: En Revista: Immunol Invest Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regulação da Expressão Gênica / Colesteatoma da Orelha Média / Infecção Persistente / Macrófagos Tipo de estudo: Observational_studies Limite: Adult / Aged / Humans / Male / Middle aged Idioma: En Revista: Immunol Invest Ano de publicação: 2021 Tipo de documento: Article