LC-MS/MS Analysis of Thiol-Containing Amino Acids in Exosomal Fraction of Serum.
J Chromatogr Sci
; 58(7): 636-640, 2020 Jul 24.
Article
em En
| MEDLINE
| ID: mdl-32577740
ABSTRACT
It has been suggested that thiol-containing amino acids could be used as biomarkers for diseases associated with oxidative stress. We investigated the thiol-containing amino acids, homocysteine (Hcy), cysteine (Cys), glutathione (GSH) and γ-glutamylcysteine (γ-GluCys), in commercial human serum by using liquid chromatography-tandem mass spectrometry (LC-MS/MS) after precolumn derivatization with 4-fluoro-7-sulfobenzofurazan. This method was applied to determine the composition of thiol-containing amino acids in exosomes prepared from the serum. Hcy, Cys, GSH and γ-GluCys could be detected in the exosomal fraction, and the ratio of each thiol-containing amino acid was similar to those in the corresponding native serum. Cys (94.76%) was most enriched in the exosomal fraction, followed by GSH (2.97%), γ-GluCys (1.59%) and Hcy (0.68%). These findings suggest that thiol-containing amino acids, Hcy, Cys, GSH and γ-GluCys, are included in exosomes in human serum.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida
/
Cisteína
/
Espectrometria de Massas em Tandem
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Exossomos
/
Glutationa
Limite:
Humans
Idioma:
En
Revista:
J Chromatogr Sci
Ano de publicação:
2020
Tipo de documento:
Article