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Shortening the sgRNA-DNA interface enables SpCas9 and eSpCas9(1.1) to nick the target DNA strand.
Fan, Rong; Chai, Zhuangzhuang; Xing, Sinian; Chen, Kunling; Qiu, Fengti; Chai, Tuanyao; Qiu, Jin-Long; Zhang, Zhengbin; Zhang, Huawei; Gao, Caixia.
Afiliação
  • Fan R; Key Laboratory of Agricultural Water Resources, Hebei Laboratory of Agricultural Water-Saving, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050022, China.
  • Chai Z; State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.
  • Xing S; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Chen K; State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.
  • Qiu F; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Chai T; State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.
  • Qiu JL; State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.
  • Zhang Z; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Zhang H; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Gao C; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
Sci China Life Sci ; 63(11): 1619-1630, 2020 Nov.
Article em En | MEDLINE | ID: mdl-32592086
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / RNA Guia de Cinetoplastídeos / Desoxirribonuclease I / Proteína 9 Associada à CRISPR Idioma: En Revista: Sci China Life Sci Ano de publicação: 2020 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / RNA Guia de Cinetoplastídeos / Desoxirribonuclease I / Proteína 9 Associada à CRISPR Idioma: En Revista: Sci China Life Sci Ano de publicação: 2020 Tipo de documento: Article