Placentaderived mesenchymal stem cells ameliorate lipopolysaccharideinduced inflammation in RAW264.7 cells and acute lung injury in rats.
Mol Med Rep
; 22(2): 1458-1466, 2020 08.
Article
em En
| MEDLINE
| ID: mdl-32626979
ABSTRACT
Acute lung injury (ALI) is a severe lung syndrome with high morbidity and mortality, due to its complex mechanism and lack of effective therapy. The use of placentaderived mesenchymal stem cells (pMSCs) has provided novel insight into treatment options of ALI. The effects of pMSCs on lipopolysaccharide (LPS)induced inflammation were studied using a coculture protocol with LPSstimulated RAW264.7 cells. An LPSinduced ALI SpragueDawley rat model was developed by intravenously injecting 7.5 mg/kg LPS, and intratracheal instillation of 1x105 pMSCs was performed after administration of LPS to investigate the therapeutic potential of these cells. pMSCs ameliorated LPSinduced ALI, as suggested by downregulated proinflammatory cytokine tumor necrosis factorα and increased antiinflammatory cytokine interleukin10 in both cell and animal models. Moreover, the protein and leukocyte cells in bronchoalveolar lavage fluid decreased at a rapid rate after treatment with pMSCs. Histopathology demonstrated that pMSCs alleviated the infiltration of inflammatory cells, pulmonary hyperemia and hemorrhage, and interstitial edema. In addition, pMSC reduced the LPSinduced expression of CXC motif chemokine ligand 12 in RAW264.7 macrophages and in lung tissue of ALI rats. This demonstrated that pMSCs are therapeutically effective in LPSinduced ALI.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
6_ODS3_enfermedades_notrasmisibles
Base de dados:
MEDLINE
Assunto principal:
Citocinas
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Transplante de Células-Tronco Mesenquimais
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Lesão Pulmonar Aguda
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Células-Tronco Mesenquimais
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Inflamação
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Pulmão
Tipo de estudo:
Guideline
/
Prognostic_studies
Limite:
Animals
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Pregnancy
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2020
Tipo de documento:
Article