Antioxidant Activity of Flavonoid Rich Fraction of Ligaria cuneifolia (Loranthaceae).
Chem Biodivers
; 17(10): e2000302, 2020 Oct.
Article
em En
| MEDLINE
| ID: mdl-32725761
ABSTRACT
Ligaria cuneifolia (Ruiz & Pav.) Tiegh. (Loranthaceae), the 'Argentine mistletoe', is a hemiparasite species largely used in folk medicine. The aim of this study was to evaluate the antioxidant activity using inâ
vitro, ex vivo, and inâ
vivo methods. A screening of phenolics was performed by UV spectroscopy on different fractions. The antioxidant capacity was evaluated inâ
vitro by the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH. ) assay on a crude extract (CE), ethyl acetate fraction (EAF), and aqueous fraction (AF). The results suggest that EAF concentrates the antioxidant capacity and was selected for further analysis. Capillary electrophoresis was employed to monitor the individual antioxidant capacity and the potential contributors to this effect. Ex vivo assays showed an efficient inhibition of tert-butyl hydroperoxide-induced rat liver phospholipid oxidation, as well as rat brain autoxidation, and H2 O2 -induced DNA damage in blood monocytes. In vivo, the topical application of EAF significantly decreased skin chemiluminescence in a mice model.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfolipídeos
/
Flavonoides
/
Extratos Vegetais
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Loranthaceae
/
Antioxidantes
Limite:
Animals
País/Região como assunto:
America do sul
/
Argentina
Idioma:
En
Revista:
Chem Biodivers
Ano de publicação:
2020
Tipo de documento:
Article