Radiation causes tissue damage by dysregulating inflammasome-gasdermin D signaling in both host and transplanted cells.
PLoS Biol
; 18(8): e3000807, 2020 08.
Article
em En
| MEDLINE
| ID: mdl-32760056
ABSTRACT
Radiotherapy is a commonly used conditioning regimen for bone marrow transplantation (BMT). Cytotoxicity limits the use of this life-saving therapy, but the underlying mechanisms remain poorly defined. Here, we use the syngeneic mouse BMT model to test the hypothesis that lethal radiation damages tissues, thereby unleashing signals that indiscriminately activate the inflammasome pathways in host and transplanted cells. We find that a clinically relevant high dose of radiation causes severe damage to bones and the spleen through mechanisms involving the NLRP3 and AIM2 inflammasomes but not the NLRC4 inflammasome. Downstream, we demonstrate that gasdermin D (GSDMD), the common effector of the inflammasomes, is also activated by radiation. Remarkably, protection against the injury induced by deadly ionizing radiation occurs only when NLRP3, AIM2, or GSDMD is lost simultaneously in both the donor and host cell compartments. Thus, this study reveals a continuum of the actions of lethal radiation relayed by the inflammasome-GSDMD axis, initially affecting recipient cells and ultimately harming transplanted cells as they grow in the severely injured and toxic environment. This study also suggests that therapeutic targeting of inflammasome-GSDMD signaling has the potential to prevent the collateral effects of intense radiation regimens.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células da Medula Óssea
/
Transplante de Medula Óssea
/
Proteínas de Ligação a Fosfato
/
Peptídeos e Proteínas de Sinalização Intracelular
/
Proteínas de Ligação a DNA
/
Inflamassomos
/
Proteína 3 que Contém Domínio de Pirina da Família NLR
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
PLoS Biol
Ano de publicação:
2020
Tipo de documento:
Article