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A novel LC-HRMS method reveals cysteinyl and glutathionyl polysulfides in wine.
van Leeuwen, Katryna A; Nardin, Tiziana; Barker, David; Fedrizzi, Bruno; Nicolini, Giorgio; Larcher, Roberto.
Afiliação
  • van Leeuwen KA; School of Chemical Sciences, The University of Auckland, 23 Symonds Street, Auckland, 1142, New Zealand. Electronic address: k.vanleeuwen@auckland.ac.nz.
  • Nardin T; Technology Transfer Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010, San Michele all' Adige, Italy. Electronic address: tiziana.nardin@fmach.it.
  • Barker D; School of Chemical Sciences, The University of Auckland, 23 Symonds Street, Auckland, 1142, New Zealand. Electronic address: d.barker@auckland.ac.nz.
  • Fedrizzi B; School of Chemical Sciences, The University of Auckland, 23 Symonds Street, Auckland, 1142, New Zealand. Electronic address: b.fedrizzi@auckland.ac.nz.
  • Nicolini G; Technology Transfer Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010, San Michele all' Adige, Italy. Electronic address: giorgio.nicolini@fmach.it.
  • Larcher R; Technology Transfer Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010, San Michele all' Adige, Italy. Electronic address: roberto.larcher@fmach.it.
Talanta ; 218: 121105, 2020 Oct 01.
Article em En | MEDLINE | ID: mdl-32797871
A novel ultra high pressure liquid chromatography combined with high resolution mass spectrometry (UHPLC-HRMS) method was developed to study glutathionyl and cysteinyl polysulfides in wine. Different HPLC columns were investigated in order to optimise the chromatographic resolution of the polysulfide standard mixtures synthesised in-house. The optimisation of the chromatographic conditions when trying to separate glutathionylated and cysteinylated species containing from 3 to 5 sulfur atoms proved particularly challenging, with the cationic exchange column IonPac CS12A-MS resulting to be the best column for this task.The synergistic application of the newly developed methods together with the synthesised reference standard mixtures allowed the identification and the detection of 11 different glutathionyl and cysteinyl polysulfides. Moreover, analysing 15 young white wines was possible to confirm the presence of GSSSG in wine (GS = glutathione). More importantly, this study allowed for the first identification of several symmetric and asymmetric new polysulfides, namely: GSSSSG, CSSSC (CS = cysteine), CSSSSC, CSSSG, and CSSSSG. These molecules have not previously been identified in wine, raising the question on their biogenesis and role on wine quality.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Talanta Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Talanta Ano de publicação: 2020 Tipo de documento: Article