Optimizing imaging speed and excitation intensity for single-molecule localization microscopy.
Nat Methods
; 17(9): 909-912, 2020 09.
Article
em En
| MEDLINE
| ID: mdl-32807954
ABSTRACT
High laser powers are common practice in single-molecule localization microscopy to speed up data acquisition. Here we systematically quantified how excitation intensity influences localization precision and labeling density, the two main factors determining data quality. We found a strong trade-off between imaging speed and quality and present optimized imaging protocols for high-throughput, multicolor and three-dimensional single-molecule localization microscopy with greatly improved resolution and effective labeling efficiency.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Processamento de Imagem Assistida por Computador
/
Imagem Individual de Molécula
Tipo de estudo:
Guideline
Limite:
Humans
Idioma:
En
Revista:
Nat Methods
Ano de publicação:
2020
Tipo de documento:
Article