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Tumor-Specific CD8+ T-Cell Responses Induced by DNA Vaccination.
Cáceres-Morgado, Pablo; Lladser, Alvaro.
Afiliação
  • Cáceres-Morgado P; Laboratory of Immunoncology, Fundación Ciencia & Vida, Santiago, Chile.
  • Lladser A; Laboratory of Immunoncology, Fundación Ciencia & Vida, Santiago, Chile. alladser@cienciavida.org.
Methods Mol Biol ; 2197: 225-239, 2021.
Article em En | MEDLINE | ID: mdl-32827140
DNA vaccines assisted by electroporation efficiently trigger antitumor cytotoxic CD8+ T cell responses in preclinical cancer models and hold potential for human use. They can be easily engineered to express either tumor-associated self-antigens, which are broadly expressed among tumor patients but also in healthy tissue, or tumor-specific neoantigens, which are uniquely expressed in tumors and differ among patients. Recently, it has been demonstrated that DNA vaccination generates both circulating and tissue-resident compartments of CD8+ T cells, which act concertedly against tumors. Here we describe the steps to obtain and test DNA vaccines against models of self-antigens and neoantigens in mice. It includes the evaluation of effector and memory CD8+ T cell responses, as well as assessing the antitumor potential in vivo using transplantable syngeneic tumor models.
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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 2_ODS3 Base de dados: MEDLINE Assunto principal: Linfócitos T CD8-Positivos / Vacinas Anticâncer / Vacinas de DNA / Especificidade do Receptor de Antígeno de Linfócitos T / Antígenos de Neoplasias Limite: Animals / Humans Idioma: En Revista: Methods Mol Biol Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 2_ODS3 Base de dados: MEDLINE Assunto principal: Linfócitos T CD8-Positivos / Vacinas Anticâncer / Vacinas de DNA / Especificidade do Receptor de Antígeno de Linfócitos T / Antígenos de Neoplasias Limite: Animals / Humans Idioma: En Revista: Methods Mol Biol Ano de publicação: 2021 Tipo de documento: Article