Facilitating islet transplantation using a three-step approach with mesenchymal stem cells, encapsulation, and pulsed focused ultrasound.
Stem Cell Res Ther
; 11(1): 405, 2020 09 18.
Article
em En
| MEDLINE
| ID: mdl-32948247
ABSTRACT
BACKGROUND:
The aim of this study was to examine the effect of a three-step approach that utilizes the application of adipose tissue-derived mesenchymal stem cells (AD-MSCs), encapsulation, and pulsed focused ultrasound (pFUS) to help the engraftment and function of transplanted islets.METHODS:
In step 1, islets were co-cultured with AD-MSCs to form a coating of AD-MSCs on islets here, AD-MSCs had a cytoprotective effect on islets; in step 2, islets coated with AD-MSCs were conformally encapsulated in a thin layer of alginate using a co-axial air-flowmethod:
here, the capsule enabled AD-MSCs to be in close proximity to islets; in step 3, encapsulated islets coated with AD-MSCs were treated with pFUS here, pFUS enhanced the secretion of insulin from islets as well as stimulated the cytoprotective effect of AD-MSCs.RESULTS:
Our approach was shown to prevent islet death and preserve islet functionality in vitro. When 175 syngeneic encapsulated islets coated with AD-MSCs were transplanted beneath the kidney capsule of diabetic mice, and then followed every 3 days with pFUS treatment until day 12 post-transplantation, we saw a significant improvement in islet function with diabetic animals re-establishing glycemic control over the course of our study (i.e., 30 days). In addition, our approach was able to enhance islet engraftment by facilitating their revascularization and reducing inflammation.CONCLUSIONS:
This study demonstrates that our clinically translatable three-step approach is able to improve the function and viability of transplanted islets.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
6_ODS3_enfermedades_notrasmisibles
Base de dados:
MEDLINE
Assunto principal:
Transplante das Ilhotas Pancreáticas
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Ilhotas Pancreáticas
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Transplante de Células-Tronco Mesenquimais
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Diabetes Mellitus Experimental
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Células-Tronco Mesenquimais
Limite:
Animals
Idioma:
En
Revista:
Stem Cell Res Ther
Ano de publicação:
2020
Tipo de documento:
Article