Quantitative analysis of subcellular distributions with an open-source, object-based tool.
Biol Open
; 9(10)2020 10 19.
Article
em En
| MEDLINE
| ID: mdl-32973081
ABSTRACT
The subcellular localization of objects, such as organelles, proteins, or other molecules, instructs cellular form and function. Understanding the underlying spatial relationships between objects through colocalization analysis of microscopy images is a fundamental approach used to inform biological mechanisms. We generated an automated and customizable computational tool, the SubcellularDistribution pipeline, to facilitate object-based image analysis from three-dimensional (3D) fluorescence microcopy images. To test the utility of the SubcellularDistribution pipeline, we examined the subcellular distribution of mRNA relative to centrosomes within syncytial Drosophila embryos. Centrosomes are microtubule-organizing centers, and RNA enrichments at centrosomes are of emerging importance. Our open-source and freely available software detected RNA distributions comparably to commercially available image analysis software. The SubcellularDistribution pipeline is designed to guide the user through the complete process of preparing image analysis data for publication, from image segmentation and data processing to visualization.This article has an associated First Person interview with the first author of the paper.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transporte Biológico
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Processamento de Imagem Assistida por Computador
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Software
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Espaço Intracelular
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Microscopia
Tipo de estudo:
Qualitative_research
Idioma:
En
Revista:
Biol Open
Ano de publicação:
2020
Tipo de documento:
Article