Your browser doesn't support javascript.
loading
In situ-forming collagen hydrogel crosslinked via multi-functional PEG as a matrix therapy for corneal defects.
Fernandes-Cunha, Gabriella Maria; Chen, Karen Mei; Chen, Fang; Le, Peter; Han, Ju Hee; Mahajan, Leela Ann; Lee, Hyun Jong; Na, Kyung Sun; Myung, David.
Afiliação
  • Fernandes-Cunha GM; Ophthalmology, Byers Eye Institute, Stanford University School of Medicine, Palo Alto, CA, USA.
  • Chen KM; Ophthalmology, Byers Eye Institute, Stanford University School of Medicine, Palo Alto, CA, USA.
  • Chen F; Ophthalmology, Byers Eye Institute, Stanford University School of Medicine, Palo Alto, CA, USA.
  • Le P; Ophthalmology, Byers Eye Institute, Stanford University School of Medicine, Palo Alto, CA, USA.
  • Han JH; Ophthalmology, Byers Eye Institute, Stanford University School of Medicine, Palo Alto, CA, USA.
  • Mahajan LA; Ophthalmology, Byers Eye Institute, Stanford University School of Medicine, Palo Alto, CA, USA.
  • Lee HJ; Chemical and Biological Engineering, Gachon University, Seongnam-si, Gyeonggi-do, Republic of Korea.
  • Na KS; Ophthalmology and Visual Science, Yeouido St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
  • Myung D; Ophthalmology, Byers Eye Institute, Stanford University School of Medicine, Palo Alto, CA, USA. djmyung@stanford.edu.
Sci Rep ; 10(1): 16671, 2020 10 07.
Article em En | MEDLINE | ID: mdl-33028837
ABSTRACT
Visually significant corneal injuries and subsequent scarring collectively represent a major global human health challenge, affecting millions of people worldwide. Unfortunately, less than 2% of patients who could benefit from a sight-restoring corneal transplant have access to cadaveric donor corneal tissue. Thus, there is a critical need for new ways to repair corneal defects that drive proper epithelialization and stromal remodeling of the wounded area without the need for cadeveric donor corneas. Emerging therapies to replace the need for donor corneas include pre-formed biosynthetic buttons and in situ-forming matrices that strive to achieve the transparency, biocompatibility, patient comfort, and biointegration that is possible with native tissue. Herein, we report on the development of an in situ-forming hydrogel of collagen type I crosslinked via multi-functional polyethylene glycol (PEG)-N-hydroxysuccinimide (NHS) and characterize its biophysical properties and regenerative capacity both in vitro and in vivo. The hydrogels form under ambient conditions within minutes upon mixing without the need for an external catalyst or trigger such as light or heat, and their transparency, degradability, and stiffness are modulated as a function of number of PEG arms and concentration of PEG. In addition, in situ-forming PEG-collagen hydrogels support the migration and proliferation of corneal epithelial and stromal cells on their surface. In vivo studies in which the hydrogels were formed in situ over stromal keratectomy wounds without sutures showed that they supported multi-layered surface epithelialization. Overall, the in situ forming PEG-collagen hydrogels exhibited physical and biological properties desirable for a corneal stromal defect wound repair matrix that could be applied without the need for sutures or an external trigger such as a catalyst or light energy.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polietilenoglicóis / Materiais Biocompatíveis / Hidrogéis / Colágeno Tipo I / Lesões da Córnea Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polietilenoglicóis / Materiais Biocompatíveis / Hidrogéis / Colágeno Tipo I / Lesões da Córnea Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2020 Tipo de documento: Article