Parallel global profiling of plant TOR dynamics reveals a conserved role for LARP1 in translation.
Elife
; 92020 10 15.
Article
em En
| MEDLINE
| ID: mdl-33054972
ABSTRACT
Target of rapamycin (TOR) is a protein kinase that coordinates eukaryotic metabolism. In mammals, TOR specifically promotes translation of ribosomal protein (RP) mRNAs when amino acids are available to support protein synthesis. The mechanisms controlling translation downstream from TOR remain contested, however, and are largely unexplored in plants. To define these mechanisms in plants, we globally profiled the plant TOR-regulated transcriptome, translatome, proteome, and phosphoproteome. We found that TOR regulates ribosome biogenesis in plants at multiple levels, but through mechanisms that do not directly depend on 5' oligopyrimidine tract motifs (5'TOPs) found in mammalian RP mRNAs. We then show that the TOR-LARP1-5'TOP signaling axis is conserved in plants and regulates expression of a core set of eukaryotic 5'TOP mRNAs, as well as new, plant-specific 5'TOP mRNAs. Our study illuminates ancestral roles of the TOR-LARP1-5'TOP metabolic regulatory network and provides evolutionary context for ongoing debates about the molecular function of LARP1.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biossíntese de Proteínas
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Proteínas de Ligação a RNA
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Arabidopsis
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Regulação da Expressão Gênica de Plantas
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Fosfatidilinositol 3-Quinases
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Proteínas de Arabidopsis
Idioma:
En
Revista:
Elife
Ano de publicação:
2020
Tipo de documento:
Article