Bioconversion of Stevioside to Rebaudioside E Using Glycosyltransferase UGTSL2.
Appl Biochem Biotechnol
; 193(3): 637-649, 2021 Mar.
Article
em En
| MEDLINE
| ID: mdl-33057971
ABSTRACT
Rebaudioside E, one of the minor components of steviol glycosides, was first isolated and identified from Stevia rebaudiana in 1977. It is a high-intensity sweetener that tastes about 150-200 times sweeter than sucrose and is also a precursor for biosynthesis of rebaudioside D and rebaudioside M, the next-generation Stevia sweeteners. In this work, new unknown steviol glycosides were enzymatically synthesized from stevioside by coupling UDP-glucosyltransferase UGTSL2 from Solanum lycopersicum and sucrose synthase StSUS1 from Solanum tuberosum. Rebaudioside E was speculated to be the main product of glucosylation of the Glc(ß1âC-19) residue of stevioside along with the formation of a (ß1â2) linkage based on the analysis of the regioselectivity and stereoselectivity of UGTSL2, and verified afterwards by LC-MS/MS with standard. In a 20-ml bioconversion reaction of 20 g/l stevioside by UGTSL2 and StSUS1, 15.92 g/l rebaudioside E was produced for 24 h.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
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Glicosiltransferases
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Solanum lycopersicum
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Diterpenos do Tipo Caurano
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Glucosídeos
Idioma:
En
Revista:
Appl Biochem Biotechnol
Ano de publicação:
2021
Tipo de documento:
Article