LINC00106 prevents against metastasis of thyroid cancer by inhibiting epithelial-mesenchymal transition.
Eur Rev Med Pharmacol Sci
; 24(19): 10015-10021, 2020 10.
Article
em En
| MEDLINE
| ID: mdl-33090406
ABSTRACT
OBJECTIVE:
Thyroid cancer (TC) is a common malignant tumor of the endocrine system, and its morbidity and mortality are in the high places. Recent studies have focused on exploring biological markers and targeted therapy for TC. This research aims to elucidate the role of LINC00106 in the progression of TC and the regulatory mechanisms. PATIENTS ANDMETHODS:
Differential level of LINC00106 in a downloaded profile containing TC and normal tissues from GEPIA database was analyzed. Subsequently, its level in TC tissues and cell lines was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The relationship between LINC00106 level and clinical data of TC patients was assessed, including age, tumor staging, lymphatic metastasis, and overall survival. After transfection of si-LINC00106, TC cell metastasis was evaluated by wound healing and transwell assay. Relative levels of E-cadherin, N-cadherin, ß-catenin, and Vimentin regulated by LINC00106 were determined using qRT-PCR and Western blot.RESULTS:
LINC00106 was downregulated in TC tissues than normal ones. Its level was correlated to tumor staging, lymphatic metastasis and overall survival in TC patients. The knockdown of LINC00106 in BCPCP and TPC-1 cells enhanced migratory and invasive abilities and triggered the process of epithelial-mesenchymal transition (EMT).CONCLUSIONS:
LINC00106 is lowly expressed in TC specimens, which attenuates migratory and invasive abilities in TC by inhibiting EMT as a tumor suppressor.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Glândula Tireoide
/
Transição Epitelial-Mesenquimal
/
RNA Longo não Codificante
Tipo de estudo:
Prognostic_studies
Limite:
Humans
/
Middle aged
Idioma:
En
Revista:
Eur Rev Med Pharmacol Sci
Ano de publicação:
2020
Tipo de documento:
Article