Assessment of intrasynovial injection in horses by contrast-enhanced ultrasonography using air bubbles created by agitation of solution.

ABSTRACT

BACKGROUND: Accuracy of intrasynovial injections can be challenging to assess in a clinical setting in horses. Contrast-enhanced ultrasonography (CEUS) using injectate agitated with air has been used to determine the success rates of synovial injections in human rheumatology. OBJECTIVES: To assess the diagnostic sensitivity and specificity of CEUS and to describe its clinical use. STUDY DESIGNS Cadaveric study followed by a prospective descriptive observational study. METHODS: Part 1 CEUS was performed following injection of agitated methylene-blue solution targeting 13 different anatomical synovial structures from three equine cadavers. Contrast was seen as hyperechoic dots, patches or lines on ultrasonography. CEUS was classified as positive if contrast was considered to be intrasynovial and negative if contrast was considered to be extrasynovial. A second synoviocentesis was performed to determine if the injection was intrasynovial based on the presence or absence of methylene-blue. Estimates of sensitivity and specificity were calculated. Part 2 CEUS was performed following injection of agitated solutions targeting synovial structures as part of routine investigation and treatment of clinical cases. RESULTS: Part 1 CEUS was correctly classified as positive or negative in all intrasynovial and extrasynovial injections respectively. The sensitivity estimate was 100% (CI 93%-100%) and the specificity estimates was 100% (CI 16%-100%). Part 2 The technique was used safely for 26 injections (14 horses; 19 different synovial structures) administered to localise or treat lameness. Traumatic intersynovial communications or synovial membrane defects were identified using CEUS in 3 horses. MAIN LIMITATIONS: The low number of extrasynovial injections in Part 1 resulted in an imprecise specificity estimate. CONCLUSIONS: In horses, CEUS performed following intended intrasynovial injection can be useful for identifying unsuccessful injections.