Your browser doesn't support javascript.
loading
Ustilago maydis effector Jsi1 interacts with Topless corepressor, hijacking plant jasmonate/ethylene signaling.
Darino, Martin; Chia, Khong-Sam; Marques, Joana; Aleksza, David; Soto-Jiménez, Luz Mayela; Saado, Indira; Uhse, Simon; Borg, Michael; Betz, Ruben; Bindics, Janos; Zienkiewicz, Krzysztof; Feussner, Ivo; Petit-Houdenot, Yohann; Djamei, Armin.
Afiliação
  • Darino M; Gregor Mendel Institute of Molecular Plant Biology (GMI), Austrian Academy of Sciences (OEAW), Vienna BioCenter (VBC), Vienna, 1030, Austria.
  • Chia KS; The Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), OT Gatersleben, 06466, Germany.
  • Marques J; Gregor Mendel Institute of Molecular Plant Biology (GMI), Austrian Academy of Sciences (OEAW), Vienna BioCenter (VBC), Vienna, 1030, Austria.
  • Aleksza D; University of Natural Resources and Life Sciences (BOKU), Vienna, 1180, Austria.
  • Soto-Jiménez LM; Gregor Mendel Institute of Molecular Plant Biology (GMI), Austrian Academy of Sciences (OEAW), Vienna BioCenter (VBC), Vienna, 1030, Austria.
  • Saado I; The Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), OT Gatersleben, 06466, Germany.
  • Uhse S; Gregor Mendel Institute of Molecular Plant Biology (GMI), Austrian Academy of Sciences (OEAW), Vienna BioCenter (VBC), Vienna, 1030, Austria.
  • Borg M; Gregor Mendel Institute of Molecular Plant Biology (GMI), Austrian Academy of Sciences (OEAW), Vienna BioCenter (VBC), Vienna, 1030, Austria.
  • Betz R; The Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), OT Gatersleben, 06466, Germany.
  • Bindics J; Gregor Mendel Institute of Molecular Plant Biology (GMI), Austrian Academy of Sciences (OEAW), Vienna BioCenter (VBC), Vienna, 1030, Austria.
  • Zienkiewicz K; Institute of Molecular Biotechnology (IMBA), Vienna BioCenter (VBC), Vienna, 1030, Austria.
  • Feussner I; Service Unit for Metabolomics and Lipidomics, Goettingen Center for Molecular Biosciences (GZMB), University of Goettingen, Goettingen, D-37077, Germany.
  • Petit-Houdenot Y; Department of Plant Biochemistry, Albrecht von Haller Institute and Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen, D-37077, Germany.
  • Djamei A; Service Unit for Metabolomics and Lipidomics, Goettingen Center for Molecular Biosciences (GZMB), University of Goettingen, Goettingen, D-37077, Germany.
New Phytol ; 229(6): 3393-3407, 2021 03.
Article em En | MEDLINE | ID: mdl-33247447
Ustilago maydis is the causal agent of maize smut disease. During the colonization process, the fungus secretes effector proteins that suppress immune responses and redirect the host metabolism in favor of the pathogen. As effectors play a critical role during plant colonization, their identification and functional characterization are essential to understanding biotrophy and disease. Using biochemical, molecular, and transcriptomic techniques, we performed a functional characterization of the U. maydis effector Jasmonate/Ethylene signaling inducer 1 (Jsi1). Jsi1 interacts with several members of the plant corepressor family Topless/Topless related (TPL/TPR). Jsi1 expression in Zea mays and Arabidopsis thaliana leads to transcriptional induction of the ethylene response factor (ERF) branch of the jasmonate/ethylene (JA/ET) signaling pathway. In A. thaliana, activation of the ERF branch leads to biotrophic susceptibility. Jsi1 likely activates the ERF branch via an EAR (ET-responsive element binding-factor-associated amphiphilic repression) motif, which resembles EAR motifs from plant ERF transcription factors, that interacts with TPL/TPR proteins. EAR-motif-containing effector candidates were identified from different fungal species, including Magnaporthe oryzae, Sporisorium scitamineum, and Sporisorium reilianum. Interaction between plant TPL proteins and these effector candidates from biotrophic and hemibiotrophic fungi indicates the convergent evolution of effectors modulating the TPL/TPR corepressor hub.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Ustilago Tipo de estudo: Prognostic_studies Idioma: En Revista: New Phytol Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Ustilago Tipo de estudo: Prognostic_studies Idioma: En Revista: New Phytol Ano de publicação: 2021 Tipo de documento: Article