lamassemble: Multiple Alignment and Consensus Sequence of Long Reads.
Methods Mol Biol
; 2231: 135-145, 2021.
Article
em En
| MEDLINE
| ID: mdl-33289891
ABSTRACT
Long DNA and RNA reads from nanopore and PacBio technologies have many applications, but the raw reads have a substantial error rate. More accurate sequences can be obtained by merging multiple reads from overlapping parts of the same sequence. lamassemble aligns up to â¼1000 reads to each other, and makes a consensus sequence, which is often much more accurate than the raw reads. It is useful for studying a region of interest such as an expanded tandem repeat or other disease-causing mutation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Software
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Sequência Consenso
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Alinhamento de Sequência
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Análise de Sequência de DNA
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Genômica
Limite:
Animals
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Humans
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2021
Tipo de documento:
Article