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Decreased bone marrow regulatory innate lymphoid cells show a distinctive miRNA profiling in aplastic anemia.
Li, Yingmei; Wan, Dingming; Guo, Rong; Wang, Fang; Han, Lijie; Zhang, Danfeng; Xing, Haizhou; Cao, Weijie; Liu, Yu; Xie, Xinsheng; Yu, Jifeng; Jiang, Zhongxing.
Afiliação
  • Li Y; Department of Hematology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
  • Wan D; Department of Hematology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
  • Guo R; Department of Hematology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
  • Wang F; Department of Hematology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
  • Han L; Department of Hematology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
  • Zhang D; Department of Hematology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
  • Xing H; Department of Hematology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
  • Cao W; Department of Hematology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
  • Liu Y; Department of Hematology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
  • Xie X; Department of Hematology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
  • Yu J; Department of Hematology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
  • Jiang Z; Department of Oncology, Academy of Medical and Pharmaceutical Sciences of Zhengzhou University, Zhengzhou, People's Republic of China.
Hematology ; 26(1): 37-42, 2021 Dec.
Article em En | MEDLINE | ID: mdl-33375909
OBJECTIVES: A new regulatory subpopulation of innate lymphoid cells (ILCs), regulatory innate lymphoid cells (ILCregs), has been identified with both innate lymphoid cells and regulatory cells characteristics. The purpose of this study is to explore ILCregs and its associated miRNAs in patients with aplastic anemia (AA) by evaluating ILCregs frequency, associated miRNA quantification, and their significance. METHODS: Using 4 color combinations of surface and intracellular antibody staining, the CD45+Lin-CD127+IL-10+ ILCregs from 30 healthy donors and 30 patients newly diagnosed with AA were measured by flow cytometry. Bone marrow cells were studied by next-generation sequence miRNAs quantification. RESULTS: Our results showed that the frequency of ILCregs in bone marrow cells from healthy donors (HD) and AA patients were 0.703 ± 0.941 and 0.171 ± 0.233%, respectively. The frequencies of ILCregs in AA patients were significantly lower than that in HD (p <0.05). miRNA detection results showed different expression patterns in the AA patient group comparing with HD. Comparing with HD, there were 52 miRNAs up-regulated and 130 miRNAs down-regulated from AA patients. Analysis of miRNAs from ILCregs associated genes demonstrated different miRNAs expression patterns between HD and AA patient. CONCLUSION: The present study demonstrated the deficiency of ILCregs and differential expression pattern of ILCregs gene-related miRNA in patients with AA. Further studies need to be done to explore the clinical significance of ILCregs and related miRNAs in patients with AA with large samples size and clinical follow-up.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Medula Óssea / Linfócitos / MicroRNAs / Anemia Aplástica Tipo de estudo: Prognostic_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Hematology Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Medula Óssea / Linfócitos / MicroRNAs / Anemia Aplástica Tipo de estudo: Prognostic_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Hematology Ano de publicação: 2021 Tipo de documento: Article