Waste soybean frying oil for the production, extraction, and characterization of cell-wall-associated lipases from Yarrowia lipolytica.
Bioprocess Biosyst Eng
; 44(4): 809-818, 2021 Apr.
Article
em En
| MEDLINE
| ID: mdl-33389167
ABSTRACT
The lipolytic yeast Yarrowia lipolytica produces cell-wall-associated lipases, namely Lip7p and Lip8p, that could have interesting properties as catalyst either in free (released lipase fraction-RLF) or cell-associated (cell-bound lipase fraction-CBLF) forms. Herein, a mixture of waste soybean frying oil, yeast extract and bactopeptone was found to favor the enzyme production. Best parameters for lipase activation and release from the cell wall by means of acoustic wave treatment were defined as 26 W/cm2 for 1 min for CBLF and 52 W/cm2 for 2 min for RLF. Optimal pH and temperature values for lipase activity together with storage conditions were similar for both the free enzyme and cell-associated one pH 7.0; T = 37 °C; and > 70% residual activity for 60 days at 4, - 4 °C and for 15 days at 30 °C.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Microbiologia Industrial
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Óleo de Soja
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Parede Celular
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Eliminação de Resíduos Líquidos
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Yarrowia
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Lipase
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Revista:
Bioprocess Biosyst Eng
Ano de publicação:
2021
Tipo de documento:
Article