Mitochondrial DNA Dynamics in Reprogramming to Pluripotency.
Trends Cell Biol
; 31(4): 311-323, 2021 04.
Article
em En
| MEDLINE
| ID: mdl-33422359
ABSTRACT
Mammalian cells, with the exception of erythrocytes, harbor mitochondria, which are organelles that provide energy, intermediate metabolites, and additional activities to sustain cell viability, replication, and function. Mitochondria contain multiple copies of a circular genome called mitochondrial DNA (mtDNA), whose individual sequences are rarely identical (homoplasmy) because of inherited or sporadic mutations that result in multiple mtDNA genotypes (heteroplasmy). Here, we examine potential mechanisms for maintenance or shifts in heteroplasmy that occur in induced pluripotent stem cells (iPSCs) generated by cellular reprogramming, and further discuss manipulations that can alter heteroplasmy to impact stem and differentiated cell performance. This additional insight will assist in developing more robust iPSC-based models of disease and differentiated cell therapies.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Mitocondrial
/
Células-Tronco Pluripotentes Induzidas
Limite:
Animals
Idioma:
En
Revista:
Trends Cell Biol
Ano de publicação:
2021
Tipo de documento:
Article