Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential.
Regen Ther
; 16: 1-11, 2021 Mar.
Article
em En
| MEDLINE
| ID: mdl-33426237
INTRODUCTION: The p75 neurotrophin receptor (p75NTR) is known as an efficient marker for the prospective isolation of mesenchymal stem cells (MSCs) and neural crest-derived stem cells (NCSCs). To date, there is quite limited information concerning p75NTR-expressing cells in umbilical cord (UC), although UC is known as a rich source of MSCs. We show for the first time the localization, phenotype, and functional properties of p75NTR+ cells in UC. METHODS: Human UC tissue sections were subjected to immunohistochemistry for MSC markers including p75NTR. Enzymatically isolated umbilical artery (UA) cells containing p75NTR+ cells were assessed for immunophenotype, clonogenic capacity, and differentiation potential. To identify the presence of neural crest-derived cells in the UA, P0-Cre/Floxed-EGFP reporter mouse embryos were used, and immunohistochemical analysis of UC tissue was performed. RESULTS: Immunohistochemical analysis revealed that p75NTR+ cells were specifically localized to the subendothelial area of the UA and umbilical vein. The p75NTR+ cells co-expressed PDGFRß, CD90, CD146, and NG2, phenotypic markers of MSCs and pericytes. Isolated UA cells possessed the potential to form neurospheres that further differentiated into neuronal and glial cell lineages. Genetic lineage tracing analysis showed that EGFP+ neural crest-derived cells were detected in the subendothelial area of UA with p75NTR immunoreactivity. CONCLUSIONS: These results show that UA tissue harbors p75NTR+ pericyte-like cells in the subendothelial area that have the capacity to form neurospheres and the potential for neurogenic differentiation. The lineage tracing data suggests the p75NTR+ cells are putatively derived from the neural crest.
ASMA, α-smooth muscle actin; BDNF, bone-derived neurotrophic factor; CFU-F, colony-forming unit fibroblast; DAPI, 4',6-diamino-2-phenylindole; DMEM, Dulbecco's modified Eagle medium; EGF, epidermal growth factor; EGFP, enhanced green fluorescent protein; EdU, 5-ethynyl-2'-deoxyuridine; FBS, fetal bovine serum; FGF-2, fibroblast growth factor-2; FSK, forskolin; GFAP, glial fibrillary acidic protein; MAP2, microtubule-associated protein 2; MSCs, mesenchymal stem cells; Mesenchymal stem cells; NCSCs, neural crest-derived stem cells; NF200, neurofilament 200; NG2, neuron-glial antigen 2; Neural crest stem cells; Neurosphere; PBS, phosphate-buffered saline; PDGF, platelet-derived growth factor; RA, all-trans-retinoic acid; TBS, Tris-buffered saline; UA, umbilical artery; UC, umbilical cord; UV, umbilical vein; Umbilical cord; WJ, Wharton's jelly; p75 neurotrophin receptor; p75NTR, p75 neurotrophin receptor; vWF, von Willebrand factor; α-MEM, alpha-modified minimum essential medium; ßME, ß-mercaptoethanol
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Regen Ther
Ano de publicação:
2021
Tipo de documento:
Article