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Neuroprotective Effect of Protaetia brevitarsis seulensis' Water Extract on Trimethyltin-Induced Seizures and Hippocampal Neurodegeneration.
Lee, Sueun; Seo, Young Hye; Song, Jun Ho; Kim, Wook Jin; Lee, Ji Hye; Moon, Byeong Cheol; Ang, Mary Jasmin; Kim, Sung Ho; Moon, Changjong; Lee, Jun; Kim, Joong Sun.
Afiliação
  • Lee S; Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju 58245, Korea.
  • Seo YH; Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju 58245, Korea.
  • Song JH; Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju 58245, Korea.
  • Kim WJ; Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju 58245, Korea.
  • Lee JH; Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju 58245, Korea.
  • Moon BC; Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju 58245, Korea.
  • Ang MJ; College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University, Gwangju 61186, Korea.
  • Kim SH; College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University, Gwangju 61186, Korea.
  • Moon C; College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University, Gwangju 61186, Korea.
  • Lee J; Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju 58245, Korea.
  • Kim JS; Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju 58245, Korea.
Int J Mol Sci ; 22(2)2021 Jan 12.
Article em En | MEDLINE | ID: mdl-33445535
This study aimed to investigate whether the Protaetia brevitarsis seulensis (PB)' water extract (PBWE) ameliorates trimethyltin (TMT)-induced seizures and hippocampal neurodegeneration. To investigate the potential neuroprotective effect of the PBWE in vitro, a lactate dehydrogenase (LDH) assay was conducted in TMT-treated primary cultures of mouse hippocampal neurons. In TMT-treated adult C57BL/6 mice, behavioral and histopathological changes were evaluated by seizure scoring and Fluoro-Jade C staining, respectively. In our in vitro assay, we observed that pretreating mice hippocampal neuron cultures with the PBWE reduced TMT-induced cytotoxicity, as indicated by the decreased LDH release. Furthermore, pretreatment with the PBWE alleviated seizures and hippocampal neurodegeneration in TMT-treated mice. The antioxidant activity of the PBWE increased in a dose-dependent manner; moreover, pretreatment with the PBWE mitigated the TMT-induced Nrf2 stimulation. In addition, six major compounds, including adenine, hypoxanthine, uridine, adenosine, inosine, and benzoic acid, were isolated from the PBWE, and among them, inosine and benzoic acid have been confirmed to have an essential antioxidative activity. In conclusion, the PBWE ameliorated TMT-induced toxicity in hippocampal neurons in both in vitro and in vivo assays, through a potential antioxidative effect. Our findings suggest that the PBWE may have pharmacotherapeutic potential in neurodegenerative diseases such as seizures or epilepsy.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Besouros / Produtos Biológicos / Fármacos Neuroprotetores / Hipocampo Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Besouros / Produtos Biológicos / Fármacos Neuroprotetores / Hipocampo Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2021 Tipo de documento: Article