Evaluating cytokine production by flow cytometry using brefeldin A in mice.
STAR Protoc
; 2(1): 100244, 2021 03 19.
Article
em En
| MEDLINE
| ID: mdl-33458706
ABSTRACT
Characterizing cytokine production in situ is important for properly understanding immunologic responses. Cytokine reporter mice are limited by the need to cross markers into various knockout backgrounds and by availability of reporters of interest. To overcome this, we utilize injection of brefeldin A into mice to enable flow cytometric analysis of in situ cytokine production during a bacterial infection. While we evaluate IFN-γ production during Burkholderia thailandensis infection, this protocol can be applied to other cytokines and other mouse models. For complete details on the use and execution of this protocol, please refer to Kovacs et al. (2020) and Liu and Whitton (2005).
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Interferon gama
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Infecções por Burkholderia
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Burkholderia
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Brefeldina A
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Citometria de Fluxo
Limite:
Animals
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2021
Tipo de documento:
Article