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relA and spoT Gene Expression is Modulated in Salmonella Grown Under Static Magnetic Field.
El May, Alya; Zouaoui, Jihen; Snoussi, Sarra; Ben Mouhoub, Ramla; Landoulsi, Ahmed.
Afiliação
  • El May A; Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences de Bizerte, Université de Carthage, 7021, Zarzouna, Tunisia. alya_elmay@yahoo.fr.
  • Zouaoui J; Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences de Bizerte, Université de Carthage, 7021, Zarzouna, Tunisia.
  • Snoussi S; Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences de Bizerte, Université de Carthage, 7021, Zarzouna, Tunisia.
  • Ben Mouhoub R; Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences de Bizerte, Université de Carthage, 7021, Zarzouna, Tunisia.
  • Landoulsi A; Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences de Bizerte, Université de Carthage, 7021, Zarzouna, Tunisia.
Curr Microbiol ; 78(3): 887-893, 2021 Mar.
Article em En | MEDLINE | ID: mdl-33515321
Virtually all bacterial species synthesize high levels of (p)ppGpp (guanosine penta- or tetraphosphate), a pleiotropic regulator of the stringent response and other stresses in bacteria. relA and spoT genes are, respectively, involved in synthesis and synthesis/biodegradation of (p)ppGpp. We aimed in this work to evaluate the impact of static magnetic field (SMF) 200 mT exposure on the expression of relA and spoT genes in Salmonella enterica Hadar. Bacteria were exposed to a SMF during 9 h, and RNA extraction was followed by reverse transcriptase polymerase chain reaction (RT-PCR). The relative quantification of mRNA expression levels using the 16S rRNA reference gene did not change during the SMF exposure. However, results showed a significant increase in gene expression for relA after 3 h of exposure (P < 0.05) and after 6 h for spoT (P < 0.05). The differential gene expression of relA and spoT could be considered as a potential stress response to a SMF exposure in Salmonella related to the production/degradation of (p)ppGpp.
Assuntos

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Regulação Bacteriana da Expressão Gênica Idioma: En Revista: Curr Microbiol Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Regulação Bacteriana da Expressão Gênica Idioma: En Revista: Curr Microbiol Ano de publicação: 2021 Tipo de documento: Article