Filaggrin expression via immunohistochemistry in basal cell carcinoma and squamous cell carcinoma.
J Cutan Pathol
; 48(7): 877-883, 2021 Jul.
Article
em En
| MEDLINE
| ID: mdl-33543489
ABSTRACT
BACKGROUND:
Filaggrin is a protein integral to the structure and function of the epidermis. Filaggrin (FLG) loss-of-function (LOF) mutations are common and increase the risk of developing atopic dermatitis (AD) and ichthyosis vulgaris (IV). Epidemiologic data suggest a link between skin cancer and AD. We examined if FLG staining pattern can be used to characterize cutaneous squamous cell carcinomas (SCC), basal cell carcinomas (BCC), and reactive squamous epithelium.METHODS:
Tissue microarrays (TMAs) were created from 196 cases of formalin-fixed paraffin-embedded (FFPE) SCC and 144 BCC cases. TMAs and sections of reactive squamous epithelium were stained with optimized anti-FLG antibody and evaluated for FLG expression (normal, abnormal, or negative).RESULTS:
FLG was absent in poorly differentiated (PD) compared to well-differentiated (WD) SCC (P < .0001) and moderately-differentiated (MD) (P = .0231) SCC, and in MD compared to WD SCC (P = .0099). Abnormal staining was significantly increased in PD compared to WD cases (P = .0039) and in MD compared to WD cases (P = .0006). Most BCC did not exhibit FLG expression (P < .05). Reactive squamous epithelium demonstrated normal, but exaggerated FLG expression.CONCLUSIONS:
Our findings demonstrate the differences in FLG expression patterns in types of keratinocyte carcinomas and their mimickers.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Cutâneas
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Imuno-Histoquímica
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Carcinoma Basocelular
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Carcinoma de Células Escamosas
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Proteínas de Filamentos Intermediários
Limite:
Aged
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Female
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Humans
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Male
Idioma:
En
Revista:
J Cutan Pathol
Ano de publicação:
2021
Tipo de documento:
Article