Discovery of Zika virus host dependency factors in trophoblasts using CRISPR/Cas9 screening.
J Virol Methods
; 290: 114085, 2021 04.
Article
em En
| MEDLINE
| ID: mdl-33545196
ABSTRACT
Emerging mosquito-borne RNA viruses cause massive health complications worldwide. The Zika virus (ZIKV), in particular, has spread dramatically since 2007 and has provoked epidemics in the Americas and the South Pacific. The lack of antiviral therapy and vaccination has focused research on the investigation of ZIKV-host interactions, in order to understand underlying molecular infection mechanisms. We have established an approach for the analysis of ZIKV host dependency factors in a human trophoblast cell line and applied genome-wide CRISPR/Cas9 knockout mutagenesis. The presented method is especially of value for the identification of factors that are essential for placental infection with the potential to serve as targets for antiviral treatment.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sistemas CRISPR-Cas
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Zika virus
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Infecção por Zika virus
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
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Screening_studies
Limite:
Animals
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Female
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Humans
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Pregnancy
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
2021
Tipo de documento:
Article