A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster.
J Antibiot (Tokyo)
; 74(5): 354-358, 2021 05.
Article
em En
| MEDLINE
| ID: mdl-33558648
ABSTRACT
Using genome mining approach, we identified a novel biosynthetic gene cluster containing trans-AT type PKS genes from Streptomyces versipellis 4083-SVS6. A bacterial artificial chromosome (BAC) clone, pKU503JL68_PN1_P10-C12, accommodating the entire biosynthetic gene cluster was obtained from a BAC library. Heterologous expression of the biosynthetic gene cluster in Streptomyces lividans TK23 led to the production of a novel polyene compound, JBIR-159. We report herein the biosynthetic gene cluster for JBIR-159, and the heterologous expression, isolation, structure determination and a brief biological activity.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Streptomyces
Idioma:
En
Revista:
J Antibiot (Tokyo)
Ano de publicação:
2021
Tipo de documento:
Article