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DMSO as new, counterintuitive excipient for freeze-drying human keratinocytes.
Rockinger, Ute; Müller, Christoph; Bracher, Franz; Funk, Martin; Winter, Gerhard.
Afiliação
  • Rockinger U; Ludwig-Maximilians-Universität München, Department of Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Munich, Germany.
  • Müller C; Ludwig-Maximilians-University Munich, Department of Pharmacy, Center for Drug Research, Munich, Germany.
  • Bracher F; Ludwig-Maximilians-University Munich, Department of Pharmacy, Center for Drug Research, Munich, Germany.
  • Funk M; QRSKIN GmbH, Friedrich-Bergius-Ring 15, Würzburg, Germany.
  • Winter G; Ludwig-Maximilians-Universität München, Department of Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Munich, Germany.
Eur J Pharm Sci ; 160: 105746, 2021 May 01.
Article em En | MEDLINE | ID: mdl-33561511
ABSTRACT
DMSO is widely used as powerful cryoprotectant for the storage and transport of frozen cells. Beyond this established application of DMSO, we could now show that it has also promising lyoprotectant effects in the field of lyophilisation of therapeutic cells. Freeze-drying of HaCaT keratinocytes in 10% HES, 5% HE and in presence of DMSO led to an increase in cell membrane integrity from 25.3 ± 2.7 % without DMSO to 41.4 ± 4.3 % with 2% DMSO, as determined by trypan blue exclusion. Interruption of the lyophilisation cycle at different sampling points showed a rapid decrease of cell membrane integrity below a critical residual moisture content. DMSO was able to stabilise cell membranes below this moisture level up to a final residual moisture content of less than 1%. Furthermore, DMSO increased the total protein content of cells after freeze-drying and subsequent SDS PAGE analysis indicated that certain abundant proteins were better preserved with the use of DMSO. Owed to its low vapour pressure, a significant part of DMSO is not removed during freeze-drying and remains as plasticiser in the lyophilised cake. However, a Tg above 60°C for 2% DMSO indicates that samples can still be stored at temperatures of 2-8°C. Also, no macroscopic or microscopic collapse can be observed by SEM or BET measurements and DMSO addition leads even to more elegant cakes with reduced cake cracking. With a better preservation of cell membranes and cellular structures, DMSO can contribute to the still unsolved problem of freeze-drying cells of higher complexity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dimetil Sulfóxido / Excipientes Limite: Humans Idioma: En Revista: Eur J Pharm Sci Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dimetil Sulfóxido / Excipientes Limite: Humans Idioma: En Revista: Eur J Pharm Sci Ano de publicação: 2021 Tipo de documento: Article