Six novel variants in the PKLR gene associated with pyruvate kinase deficiency in Argentinian patients.
Clin Biochem
; 91: 26-30, 2021 May.
Article
em En
| MEDLINE
| ID: mdl-33631127
ABSTRACT
BACKGROUND:
Pyruvate kinase deficiency (PKD) is a rare recessive congenital hemolytic anemia caused by mutations in the PKLR gene. The disease shows a marked variability in clinical expression. We studied the molecular features of nine unrelated Argentinian patients with congenital hemolytic anemia associated with erythrocyte pyruvate kinase deficiency. DESIGN ANDMETHODS:
Routine hematologic investigations were performed to rule out other causes of chronic hemolytic anemia. Sanger sequencing and in-sílico analysis were carried out to identify and characterize the genetics variants.RESULTS:
Six different novel missense variants were detected among the 18 studied alleles c.661 G > C (Asp221His), c.956 G > T (Gly319Val), c.1595 G > C (Arg532Pro), c.347 G > A (Arg116Gln), c.1232 G > T (Gly411Val), c.1021G > A (Gly341Ser). Structural implications of amino-acid substitutions were correlated with the clinical phenotypes seen in the probands.CONCLUSIONS:
This is the first comprehensive report on molecular characterization of pyruvate kinase deficiency in Argentina and the second from South America that would contribute to our knowledge on the distribution and frequency of PKLR variants in our population but also offer new insights into the interpretation of the effect of PKLR variants and phenotype.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Piruvato Quinase
/
Erros Inatos do Metabolismo dos Piruvatos
/
Mutação de Sentido Incorreto
/
Alelos
/
Anemia Hemolítica Congênita não Esferocítica
Tipo de estudo:
Risk_factors_studies
Limite:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
País/Região como assunto:
America do sul
/
Argentina
Idioma:
En
Revista:
Clin Biochem
Ano de publicação:
2021
Tipo de documento:
Article