A tissue culture assay for tetrodotoxin, saxitoxin and related toxins.
Toxicon
; 26(2): 191-7, 1988.
Article
em En
| MEDLINE
| ID: mdl-3363566
ABSTRACT
In the presence of ouabain, veratridine enhances sodium influx in the mouse neuroblastoma cell line Neuro-2A (ATCC, CCL131), causing cellular swelling and subsequent death. Tetrodotoxin (puffer fish toxin) or saxitoxin (paralytic shellfish poison), both of which block the sodium channel of excitable membranes, antagonize this effect, enabling cell growth to continue. This phenomenon was used as the basis of a new assay for these toxins. It is also possible to estimate the quantity of TTX from the relationship between TTX concentration and percentage of living cells. This new method is simple, inexpensive, and sensitive, and may replace the conventional mouse bioassay.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saxitoxina
/
Tetrodotoxina
/
Toxinas Marinhas
Limite:
Animals
Idioma:
En
Revista:
Toxicon
Ano de publicação:
1988
Tipo de documento:
Article